Coding
Part:BBa_K1739003:Design
Designed by: Natalie Sloan Group: iGEM15_Kent (2015-09-15)
Sequence coding for Envirowire: Sup35NM with N-terminal CsgAss and C-terminal cytb562
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 718
Design Notes
This BioBrick has been optimised for use in the VS45 strain of E.coli containing deletions that prevent amyloid from binding to the outside of the cell and increase the rate of protein exiting the cell via the curli export system.
Source
It comes from a genomic sequence of Saccharomyces cerevisiae (bakers yeast).